Journal: STAR Protocols

Article Title: Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer’s disease

doi: 10.1016/j.xpro.2022.101149

Figure Lengend Snippet: Morphological changes in the cytoskeleton of dysfunctional VSMCs Scale bar: 20 μm

Article Snippet: Culture medium ready-to-use with FBS and antibiotics (VSMCs) , Cell Applications , Cat#311-500.

Techniques:

Journal: STAR Protocols

Article Title: Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer’s disease

doi: 10.1016/j.xpro.2022.101149

Figure Lengend Snippet: Schematic workflow supernatant assay Immunofluorescence staining shows microglia activation after incubation with supernatant from dysfunctional VSMCs. Activated microglial were characterized by morphologic changes and elevated expression of IL-6 at 100 × magnification, scale bars: 5 μm

Article Snippet: Culture medium ready-to-use with FBS and antibiotics (VSMCs) , Cell Applications , Cat#311-500.

Techniques: Immunofluorescence, Staining, Activation Assay, Incubation, Expressing

Journal: STAR Protocols

Article Title: Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer’s disease

doi: 10.1016/j.xpro.2022.101149

Figure Lengend Snippet:

Article Snippet: Culture medium ready-to-use with FBS and antibiotics (VSMCs) , Cell Applications , Cat#311-500.

Techniques: Recombinant, Wound Healing Assay, Staining, SYBR Green Assay, Software, Real-time Polymerase Chain Reaction, Microscopy, Sterility, Inverted Microscopy, Confocal Microscopy

Journal: Cell reports

Article Title: TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling

doi: 10.1016/j.celrep.2022.110395

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: For flow cytometry, hESCs were fixed with 2-4% PFA, permeabilized with methanol, and then stained with anti-SOX2 (APC; Miltenyi Biotec), anti-OCT3/4 (PE; Miltenyi Biotec), and anti-NANOG (BV421; BioLegend) antibodies and DAPI.

Techniques: Recombinant, Staining, Plasmid Preparation, Selection, DNA HS Assay, CRISPR, Software, Imaging, Inverted Microscopy, Microscopy, Flow Cytometry